Please use this identifier to cite or link to this item:
https://hdl.handle.net/20.500.12202/4142
Title: | Co-culture Activation of MAP Kinase in Drosophila S2 Cells. |
Authors: | Steinhauer, Josefa 0000-0002-0960-5271 |
Keywords: | MAP kinase ERK dp-ERK S2 cells Co-culture Western blot |
Issue Date: | 2017 |
Publisher: | Elsevier |
Citation: | Steinhauer, J. (2017). Co-culture Activation of MAP Kinase in Drosophila S2 Cells. Methods in Molecular Biology. 1487: 235-241. |
Abstract: | Intercellular communication often involves phosphorylation of signal transduction proteins, including mitogen-activated protein kinases (MAPKs). Immunological detection of phosphorylated MAPK can be used to monitor signaling in vivo, identify novel pathway components, and assess ligand activity. In this chapter, I describe a cell co-culture method to assess activity of cell-bound extracellular ligands that result in phosphorylation of the ERK (extracellular signal-regulated kinase) MAPK in Drosophila. This protocol may be adaptable to other pathways and/or model systems. |
Description: | Author's post-print PDF: Published in final edited form as: Methods Mol Biol. 2017 ; 1487: 235–241. doi:10.1007/978-1-4939-6424-6_17. |
URI: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5588906/pdf/nihms901683.pdf https://hdl.handle.net/20.500.12202/4142 |
ISSN: | 1064-3745 |
Appears in Collections: | Yeshiva College: Faculty Publications |
Files in This Item:
File | Description | Size | Format | |
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Steinhauer Co_culture MMB postp 2017nihms901683.pdf | Author's manuscript | 306.5 kB | Adobe PDF | View/Open |
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