Intercellular communication often involves phosphorylation of signal transduction proteins,
including mitogen-activated protein kinases (MAPKs). Immunological detection of
phosphorylated MAPK can be used to monitor signaling in vivo, identify novel pathway
components, and assess ligand activity. In this chapter, I describe a cell co-culture method to
assess activity of cell-bound extracellular ligands that result in phosphorylation of the ERK
(extracellular signal-regulated kinase) MAPK in
Drosophila. This protocol may be adaptable to other pathways and/or model systems.
Author's post-print PDF:
Published in final edited form as:
Methods Mol Biol. 2017 ; 1487: 235–241. doi:10.1007/978-1-4939-6424-6_17.