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dc.contributor.authorSteinhauer, Josefa
dc.identifier.citationSteinhauer, J. (2017). Co-culture Activation of MAP Kinase in Drosophila S2 Cells. Methods in Molecular Biology. 1487: 235-241.en_US
dc.descriptionAuthor's post-print PDF: Published in final edited form as: Methods Mol Biol. 2017 ; 1487: 235–241. doi:10.1007/978-1-4939-6424-6_17.en_US
dc.description.abstractIntercellular communication often involves phosphorylation of signal transduction proteins, including mitogen-activated protein kinases (MAPKs). Immunological detection of phosphorylated MAPK can be used to monitor signaling in vivo, identify novel pathway components, and assess ligand activity. In this chapter, I describe a cell co-culture method to assess activity of cell-bound extracellular ligands that result in phosphorylation of the ERK (extracellular signal-regulated kinase) MAPK in Drosophila. This protocol may be adaptable to other pathways and/or model systems.en_US
dc.description.sponsorshipAcknowledgments I thank Jean-Yves Roignant and Kevin Legent for help developing the ERK activation assay; Benny Shilo for the D2F cells; Jessica Treisman for support during the development of the protocol and critical comments on the manuscript. This work was supported by the March of Dimes Birth Defects Foundation (to Jessica Treisman) and the National Institutes of Health (grant number EY13777 to Jessica Treisman from the National Eye Institute)en_US
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
dc.subjectMAP kinaseen_US
dc.subjectS2 cellsen_US
dc.subjectWestern bloten_US
dc.titleCo-culture Activation of MAP Kinase in Drosophila S2 Cells.en_US

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