Show simple item record

dc.contributor.authorGreenberg, Nili
dc.date.accessioned2018-11-08T21:17:31Z
dc.date.available2018-11-08T21:17:31Z
dc.date.issued2017-04
dc.identifier.urihttps://hdl.handle.net/20.500.12202/4184
dc.identifier.urihttps://yulib002.mc.yu.edu/login?url=https://repository.yu.edu/handle/20.500.12202/4184
dc.descriptionThe file is restricted for YU community access only.
dc.description.abstractThe mitochondria are the cellular organelles that is responsible for respiration in eukaryotic, aerobic organisms. The enzyme ATP synthase is the site of oxidative phosphorylation, the generation of energy for cellular use in the form of ATP. The structure and location of this enzyme within the mitochondria and the cell is vital to its function. However, the crowded cellular environment prevents traditional methods of microscopy structural analysis from effectively gathering information about molecular structures in situ. Rather than existing as single monomers, ATP synthase adopts a dimeric conformation when found in its natural location, along the inner membrane of a mitochondrion. The use of cryoelectron tomography, therefore, is crucial in the quest to learn about this enzyme. When combined with computational analyses such as subtomogram averaging, this method can resolve the structure of macromolecular complexes like ATP synthase. In this investigation, we modified and optimized existing techniques of cryo-ET and subtomogram averaging to determine the structure of mitochondrial ATP synthase in insulin producing pancreatic cells.en_US
dc.description.sponsorshipS. Daniel Abraham Honors Programen_US
dc.language.isoen_USen_US
dc.publisherStern College for Womenen_US
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
dc.subjectAdenosine triphosphatase.en_US
dc.subjectTomography.en_US
dc.subjectMitochondria.en_US
dc.subjectStructure-activity relationships (Biochemistry)en_US
dc.titleDetermination of the mitochondrial ATP synthase structure by cryo-electron tomography and subtomogram averaging.en_US
dc.typeThesisen_US


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivs 3.0 United States
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 United States