dc.contributor.author | Brander, Tehilla | |
dc.date.accessioned | 2018-11-12T21:35:30Z | |
dc.date.available | 2018-11-12T21:35:30Z | |
dc.date.issued | 2014-05 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12202/4241 | |
dc.identifier.uri | https://yulib002.mc.yu.edu/login?url=https://repository.yu.edu/handle/20.500.12202/4241 | |
dc.description | The file is restricted for YU community access only. | |
dc.description.abstract | According to the National Institute of Health, half of women and a quarter of men
will break a bone due to osteoporosis, a disease categorized by low bone density. Bone
density and strength are regulated by two types of cells, osteoclasts, which dissolve bone, and
osteoblasts which build bone. If the balance between osteoclasts and osteoblasts is disturbed,
a diseased state will occur. While osteoporosis is caused by a relative increase in osteoclast
activity, osteopetrosis, a genetic disease categorized by extremely high bone density, is
caused by a decrease in osteoclast activity. It has been suggested that environmental factors
may influence osteoclast and osteoblast function. Through the use of a variety of chemical
compounds, one can endeavor to manipulate the transcription or post-transcriptional
modification of RNA transcripts critical to the functioning of osteoclasts, thereby
compromising osteoclast function and enhancing bone density. HL-60 and THP1 cell lines
were treated with various nutraceuticals and screened for possible transcriptional/posttranscriptional
changes in gene-encoded transcripts critical to osteoclast function. Both RTPCR
and qRT-PCR analysis has demonstrated that treatment of cells with extracts generated
from Gymnema Sylvestre results in a post-transcriptional modification that results in the
generation of an alternatively spliced transcript form of the transcript encoded by the RANK
gene. As mutations in the RANK gene that compromise the functionality of this gene
product have been demonstrated to cause osteopetrosis, through its effect on osteoclast
differentiation and survival, it is believed that Gymnema Sylvestre extract may be able to
compromise osteoclast function and be used as a treatment for osteoporosis. | en_US |
dc.description.sponsorship | S. Daniel Abraham Honors Program | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Stern College for Women | en_US |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 United States | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/us/ | * |
dc.subject | Asclepiadaceae --Therapeutic use. | en_US |
dc.subject | RNA. | en_US |
dc.subject | Osteoporosis --Treatment. | en_US |
dc.subject | Osteoporosis --Prevention. | en_US |
dc.subject | Herbs --Therapeutic use. | en_US |
dc.subject | Materia medica, Vegetable. | en_US |
dc.title | Effects of Gymnema Sylvestre on the post-transcriptional processing of the RANK -encoded RNA: A Possible Treatment for Osteoporosis | en_US |
dc.type | Thesis | en_US |