• Login as Editor
    View Item 
    •   Yeshiva Academic Institutional Repository
    • Albert Einstein College of Medicine (AECOM)
    • Albert Einstein College of Medicine: Doctoral Dissertations
    • View Item
    •   Yeshiva Academic Institutional Repository
    • Albert Einstein College of Medicine (AECOM)
    • Albert Einstein College of Medicine: Doctoral Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Helios regulates interleukin-2 expression and suppressive function in natural regulatory T cells

    Thumbnail

    Date
    2012
    Author
    Baine, Ian
    Metadata
    Show full item record
    Share
    Abstract
    The critical role of regulatory T cells in maintaining immune tolerance and preventing autoimmune disease has been well established. Treg cells express the transcription factor Foxp3, which acts as a master regulator of their differentiation and controls their capacity to suppress T cell responses. Treg cells have an intrinsically anergic phenotype and do not produce IL-2 or proliferate upon stimulation ex vivo. Recent reports have identified that Helios, a member of the Ikaros family of transcription factors, is expressed in Treg cells. However, its specific function(s) are not yet fully understood. In this study, we show that Helios regulates IL-2 production in Treg cells by suppressing //2 transcription. Loss of Helios in Treg cells breaks their anergic phenotype and results in de-repression of the //2 locus, allowing Treg cells to display increased baseline proliferation, and to produce IL-2 following stimulation. Conversely, forced expression of Helios in CD4 +Foxp3- Th1 cells results in a loss of their normal ability to produce IL-2. Helios acts by directly binding the //2 promoter and inducing histone deacetylation. We also show that loss of Helios in Treg cells results in decreased Foxp3 binding to the //2 promoter, indicating that Helios is a necessary permissive factor for Foxp3 binding to the //2 promoter. Interestingly, the loss of Helios in Treg cells also causes a decrease in the suppressive capacity of Treg cells. Our results identify Helios as a key regulator of IL-2 expression in Treg cells, contributing to the maintenance of their anergic phenotype.
    Permanent Link(s)
    https://ezproxy.yu.edu/login?url=http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqm&rft_dat=xri:pqdiss:3536054
    https://hdl.handle.net/20.500.12202/1365
    Citation
    Source: Dissertation Abstracts International, Volume: 74-06(E), Section: B.;Advisors: Fernando Macian-Juan Committee members: Barbara Birshtein; Stefan Feske; Joseph Locker; Michael Prystowsky; Xingxing Zang.
    *This is constructed from limited available data and may be imprecise.
    Collections
    • Albert Einstein College of Medicine: Doctoral Dissertations [1674]

    Yeshiva University Libraries copyright © 2021  DuraSpace
    YAIR Self-Deposit | YAIR User's Guide | Take Down Policy | Contact Us
    Yeshiva University
     

     

    Browse

    AllCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    Login as Editor

    Statistics

    View Usage Statistics

    Yeshiva University Libraries copyright © 2021  DuraSpace
    YAIR Self-Deposit | YAIR User's Guide | Take Down Policy | Contact Us
    Yeshiva University