• Login as Editor
    View Item 
    •   Yeshiva Academic Institutional Repository
    • Albert Einstein College of Medicine (AECOM)
    • Albert Einstein College of Medicine: Doctoral Dissertations
    • View Item
    •   Yeshiva Academic Institutional Repository
    • Albert Einstein College of Medicine (AECOM)
    • Albert Einstein College of Medicine: Doctoral Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Distinct 3-O-sulfated heparan sulfate modification patterns are required for kal-1 dependent neurite branching in a context-dependent manner in C. elegans

    Thumbnail
    Date
    2013
    Author
    Tecle, Eillen
    Metadata
    Show full item record
    Abstract
    Heparan sulfate (HS) is an un-branched glycosaminoglycan exhibiting substantial molecular diversity due to multiple, non-uniformly introduced modifications including sulfations, epimerization and acetylation. HS modifications serve specific and instructive roles in neuronal development leading to the hypothesis of a HS code that regulates nervous system patterning. While the in vivo roles of many of the HS modifications have been investigated, very little is known about the function of HS 3- O-sulfation in vivo. By examining patterning of the C. elegans nervous system in loss of function mutants of the two 3-O-sulfotransferases, hst-3.1 and hst-3.2, HS 3-O-sulfation was found to be largely dispensable for overall neural development. However, generation of stereotypical axonal branches in hermaphroditic specific neurons (HSN) required hst-3.1, hst-3.2 as well as an extracellular cell adhesion molecule encoded by kal-1, the homolog of Kallmann Syndrome associated gene 1/anosmin-1. In contrast, kal-1 dependent neurite branching in AIY neurons required catalytic activity of hst-3.2 but not hst-3.1. The context dependent requirement for hst-3.2 and hst-3.1 indicates that both enzymes generate distinct types of HS modification patterns in different cell types, which regulate kal-1 to promote neurite branching. HS 3-O-sulfation does not play a general role in establishing the HS code in C. elegans, but rather plays a specialized role in a context dependent manner to establish defined aspects of neuronal circuits.
    Permanent Link(s)
    https://yulib002.mc.yu.edu/login?url=http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqm&rft_dat=xri:pqdiss:3570002
    https://hdl.handle.net/20.500.12202/1400
    Collections
    • Albert Einstein College of Medicine: Doctoral Dissertations [1674]

    Yeshiva University Libraries copyright © 2021  DuraSpace
    YAIR Self-Deposit | YAIR User's Guide | Take Down Policy | Contact Us
    Yeshiva University
     

     

    Browse

    AllCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    Login as Editor

    Statistics

    View Usage Statistics

    Yeshiva University Libraries copyright © 2021  DuraSpace
    YAIR Self-Deposit | YAIR User's Guide | Take Down Policy | Contact Us
    Yeshiva University