• Login as Editor
    View Item 
    •   Yeshiva Academic Institutional Repository
    • Albert Einstein College of Medicine (AECOM)
    • Albert Einstein College of Medicine: Doctoral Dissertations
    • View Item
    •   Yeshiva Academic Institutional Repository
    • Albert Einstein College of Medicine (AECOM)
    • Albert Einstein College of Medicine: Doctoral Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    MOLECULAR STUDIES OF THE DISSOCIATION OF COMMITMENT FROM BIOCHEMICAL DIFFERENTIATION IN A VARIANT MYOBLAST CELL LINE AND THE CHARACTERIZATION OF THE RAT SARCOMERIC MYOSIN HEAVY CHAIN MULTIGENE FAMILY

    Thumbnail
    Date
    1982
    Author
    NGUYEN, HANH THI
    Metadata
    Show full item record
    Abstract
    This study has focused on two major aspects. (1) The characterization of the myosin heavy chain (MHC) genes and how the structures of such genes facilitate their differential expression during muscle development. (2) The elucidation of the relationship between the two key events that lead to the terminally differentiated muscle phenotype: commitment and expression of the muscle-specific genes.;Using a cDNA clone containing embryonic MHC mRNA sequences, we have demonstrated by nucleic acid hybridization assays that the rat sarcomeric MHC is coded by a multigene family with shared sequence homology among the members. Nucleic acid hybridization and heteroduplex mapping studies of MHC genomic sequences have shown that there are coding regions which are conserved laterally and evolutionarily, that are interspersed with other coding sequences and intervening sequences that are tissue-specific. Additionally, these MHC gene sequences do not undergo rearrangement or amplification during muscle development and the embryonic MHC gene which is expressed in the L6E9 cells is transcribed as a larger nuclear RNA precursor. The study has unraveled structural similarities and differences among the tissue-specific genes which may play a role in their differential expression.;A variant myoblast cell line has been determined to be temperature-sensitive for commitment and fusion as determined from studies which include cloning assays, {lcub}3H{rcub}thymidine uptake and flow microfluorometry studies. At the temperature non-permissive for commitment, the 3b-2 cells synthesize and accumulate muscle-specific mRNAs to normal levels. Transcriptional activation of the muscle-specific genes is thus produced by processes that can be dissociated from commitment to terminal differentiation. However, in the absence of commitment, the induction of the muscle-specific genes is reversible and upon growth stimulation, the synthesis and accumulation of the muscle-specific mRNAs is decreased. The terminally differentiated muscle phenotype therefore requires the coupling of both the commitment event and the expression of the muscle-specific genes.
    Permanent Link(s)
    https://yulib002.mc.yu.edu/login?url=http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqm&rft_dat=xri:pqdiss:8218735
    https://hdl.handle.net/20.500.12202/2772
    Collections
    • Albert Einstein College of Medicine: Doctoral Dissertations [1674]

    Yeshiva University Libraries copyright © 2021  DuraSpace
    YAIR Self-Deposit | YAIR User's Guide | Take Down Policy | Contact Us
    Yeshiva University
     

     

    Browse

    AllCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    Login as Editor

    Statistics

    View Usage Statistics

    Yeshiva University Libraries copyright © 2021  DuraSpace
    YAIR Self-Deposit | YAIR User's Guide | Take Down Policy | Contact Us
    Yeshiva University