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dc.contributor.authorSACCA, ROSALBA
dc.date.accessioned2018-07-12T18:24:12Z
dc.date.available2018-07-12T18:24:12Z
dc.date.issued1986
dc.identifier.citationSource: Dissertation Abstracts International, Volume: 47-06, Section: B, page: 2273.
dc.identifier.urihttps://yulib002.mc.yu.edu/login?url=http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqm&rft_dat=xri:pqdiss:8621237
dc.identifier.urihttps://hdl.handle.net/20.500.12202/3124
dc.description.abstractThe development of mature blood cells is dependent on a population of precursor cells which are stimulated to proliferate and differentiate by specific regulatory proteins. The colony stimulating factor, CSF-1, can stimulate the proliferation, differentiation and survival of cells of the mononuclear phagocytic lineage. The pleiotropic response to CSF-1 is mediated via a 165 kDa cell surface receptor with an associated tyrosine kinase activity. The v-fms oncogene product of the Susan McDonough strain of feline sarcoma virus (SM-FeSV) is derived from the cellular proto-oncogene c-fms. The feline c-fms-encoded protein is a glycoprotein of 170 kDa. It is found on mature cat macrophages and has an associated tyrosine kinase activity.;Rabbit antisera to a recombinant v-fms-encoded polypeptide precipitated the feline c-fms product and cross-reacted with a 165 kDa glycoprotein from mouse macrophages. This putative murine c-fms gene product specifically bound murine CSF-1 and in the presence of the growth factor was phosphorylated on tyrosine residues. These data suggest that the murine c-fms proto-oncogene product and the CSF-1 receptor are functionally and antigenically related molecules.;Cells transformed by the Susan McDonough strain of the feline sarcoma virus (SM-FeSV) express a v-fms-encoded glycoprotein at the cell surface which specifically binds murine CSF-1. A monoclonal antibody to a v-fms-encoded epitope can compete for this binding and chemical cross-linking demonstrated that CSF-1 is bound to the v-fms gene product. The SM-FeSV lines were found to secrete CSF-1, but growth of the transformed cells was not inhibited by antibodies to the v-fms gene product or to the growth factor. Tyrosine phosphorylation of the v-fms is observed in the absence of CSF-1 and could not be enhanced by addition of the growth factor. These data further support the hypothesis that the c-fms proto-oncogene product is related, if not identical, to the CSF-1 receptor, and suggest that the v-fms-encoded kinase functions in the absence of an exogenous growth factor.
dc.publisherProQuest Dissertations & Theses
dc.subjectBiology.
dc.titleTHE CSF-1 RECEPTOR IS RELATED TO THE C-FMS PROTO-ONCOGENE PRODUCT (GROWTH FACTORS, RECEPTORS, TYROSINE KINASES, PHOSPHORYLATION)
dc.typeDissertation


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