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dc.contributor.authorBresnick, Anne R.
dc.date.accessioned2018-07-12T18:34:47Z
dc.date.available2018-07-12T18:34:47Z
dc.date.issued1991
dc.identifier.citationSource: Dissertation Abstracts International, Volume: 52-05, Section: B, page: 2535.;Advisors: John S. Condeelis.
dc.identifier.urihttps://yulib002.mc.yu.edu/login?url=http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqm&rft_dat=xri:pqdiss:9130398
dc.identifier.urihttps://hdl.handle.net/20.500.12202/3379
dc.description.abstractProteolysis experiments of ABP-120 from Dictyostelium discoideum demonstrate that removal of residues 89-115 from a 17-kDa tryptic peptide which retains actin binding activity, abolishes actin binding. Antibodies made against a synthetic peptide of this 27 amino acid sequence (27mer) specifically immunoprecipitate native ABP-120 from Dictyostelium high speed supernatants, demonstrating that at least a portion of the 27mer sequence is on the surface of the molecule as expected for an active site. ABP-120 is also inhibited in its binding to F-actin by Fab{dollar}\sp\prime{dollar} fragments of the anti-27mer IgG. Half-maximal inhibition occurs at an approximate molar ratio of 7 Fab{dollar}\sp\prime{dollar} fragments per ABP-120 monomer. Viscoelastic measurements indicate that ABP-120 forms fewer cross-links with F-actin in the presence of the 27mer synthetic peptide than in its absence. In F-actin cosedimentation assays, the binding of ABP-120 to actin is inhibited by the 27mer synthetic peptide, as well. In addition, the 27mer synthetic peptide cosediments with F-actin, whereas a control hydrophobic peptide and a synthetic peptide of residues 69-88 of ABP-120 do not cosediment with F-actin. These observations suggest a direct involvement of the 27mer sequence in the actin binding activity of ABP-120. The 27mer sequence is highly conserved in a number of diverse proteins including fimbrin/plastin, {dollar}\alpha{dollar}-actinin, {dollar}\beta{dollar}-spectrin, dystrophin and actin binding protein (ABP-280/non-muscle filamin). Given the conservation of the 27mer sequence in a number of actin cross-linking proteins, we suggest that the 27mer sequence is an archetypical binding site for a class of proteins which bind to the sides of actin filaments.
dc.publisherProQuest Dissertations & Theses
dc.subjectBiochemistry.
dc.titleAnalysis of the actin binding site of ABP-120 from Dictyostelium discoideum
dc.typeDissertation


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