Phospholipid-derived Signaling Molecules in Drosophila Development
MetadataShow full item record
Fatty acids are precursors of potent lipid signaling molecules. They are stored in membrane phospholipids and released by phospholipase A2s (PLA2s). Lysophospholipid acyltransferases (ATs) oppose PLA2s by re-esterifying fatty acids into phospholipids, in a biochemical pathway known as the Lands Cycle (shown in figure 1). Drosophila Lands Cycle ATs Oys and Nes, as well as seven predicted PLA2 genes, are expressed in the male reproductive tract (see figure 2). As shown in Ben-David et al. 2015, Oys and Nes are required for spermatid individualization1 . Individualization, which occurs after terminal differentiation, invests each spermatid in its own plasma membrane and removes the bulk of the cytoplasmic contents. In previous experiments, the Steinhauer lab developed a quantitative assay to measure individualization defects and demonstrated that this process is highly abnormal in oys nes double mutants1 . This process also is disrupted by mutations in the cyclooxygenase Pxt, which converts fatty acids into prostaglandin signaling molecules, but not by other phospholipid metabolism pathway mutants2 (figure 1). Together, these results suggest that fatty acid signaling controls spermatid individualization. Following these results, we set out to explore if reducing PLA2 activity (specifically iPLA2) would have similar effects on spermatogenesis and/or other aspects of development.
The file is restricted for YU community access only.
The file is restricted, or can be viewed by YU Community Only.
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 United States
Showing items related by title, author, creator and subject.
A nuclease from Drosophila and rRNA processing in yeast: Part~I. Nuclease III from adult flies of Drosophila melanogaster. Part~II. Mutants involved in pre- rRNA processing in Saccharomyces cerevisiae Shuai, Ke (ProQuest Dissertations & Theses, 1990)Part I. An endo-exonuclease (designated nuclease III) has been purified to virtual homogeneity from adult flies of Drosophila melanogaster. The enzyme degrades single- and double-stranded DNA and RNA. It has a sedimentation ...
Steinhauer, Josefa; Kondo, Shu; Vedanayagam, Jeffrey; Mohammed, Jaaved; Eizadshenass, Sogol; Kan, Lijuan; Pang, Nan; Aradhya, Rajaguru; Siepel, Adam; Lai, Eric C. (Cold Spring Harbor Laboratory Press, 2017-09-15)Relatively little is known about the in vivo functions of newly emerging genes, especially in metazoans. Although prior RNAi studies reported prevalent lethality among young gene knockdowns, our phylogenomic analyses reveal ...
Steinhauer, Josefa (Elsevier, 2017)Intercellular communication often involves phosphorylation of signal transduction proteins, including mitogen-activated protein kinases (MAPKs). Immunological detection of phosphorylated MAPK can be used to monitor ...