Co-culture Activation of MAP Kinase in Drosophila S2 Cells.
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Author's post-print PDF: Published in final edited form as: Methods Mol Biol. 2017 ; 1487: 235–241. doi:10.1007/978-1-4939-6424-6_17.
Intercellular communication often involves phosphorylation of signal transduction proteins, including mitogen-activated protein kinases (MAPKs). Immunological detection of phosphorylated MAPK can be used to monitor signaling in vivo, identify novel pathway components, and assess ligand activity. In this chapter, I describe a cell co-culture method to assess activity of cell-bound extracellular ligands that result in phosphorylation of the ERK (extracellular signal-regulated kinase) MAPK in Drosophila. This protocol may be adaptable to other pathways and/or model systems.
Steinhauer, J. (2017). Co-culture Activation of MAP Kinase in Drosophila S2 Cells. Methods in Molecular Biology. 1487: 235-241.
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