Co-culture Activation of MAP Kinase in Drosophila S2 Cells.
:0000-0002-0960-5271Description
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Methods Mol Biol. 2017 ; 1487: 235–241. doi:10.1007/978-1-4939-6424-6_17.
Abstract
Intercellular communication often involves phosphorylation of signal transduction proteins,
including mitogen-activated protein kinases (MAPKs). Immunological detection of
phosphorylated MAPK can be used to monitor signaling in vivo, identify novel pathway
components, and assess ligand activity. In this chapter, I describe a cell co-culture method to
assess activity of cell-bound extracellular ligands that result in phosphorylation of the ERK
(extracellular signal-regulated kinase) MAPK in
Drosophila. This protocol may be adaptable to other pathways and/or model systems.
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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5588906/pdf/nihms901683.pdfhttps://hdl.handle.net/20.500.12202/4142
Citation
Steinhauer, J. (2017). Co-culture Activation of MAP Kinase in Drosophila S2 Cells. Methods in Molecular Biology. 1487: 235-241.
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