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The directed movement of particles down a temperature gradient, a phenomenon known as
thermophoresis, has long been observed and recorded. However, only recently has thermophoresis been
put to use in the laboratory for a variety of biophysical applications. The most prominent and successful
of these applications include detection of biochemical interactions, analysis of molecular thermal
denaturation, and the study of the origin of biomolecules within hydrothermal pores. To detect
biochemical interactions, microscale thermophoresis (MST) is performed by setting up a temperature
gradient in solution and detecting changes in thermal diffusion induced by binding events. To analyze
thermal denaturation of molecules, the molecular movement along a temperature gradient is compared at
a range of temperatures and a change in the molecular movement is detected at the melting temperature.
Finally, thermophoresis provides insight into the origin of life as it offers a putative explanation for the
accumulation and replication of pre-biotic molecules. These novel applications of thermophoresis, at the
intersection of the physical and biological sciences, have unique advantages compared to other techniques
and hold great potential in the study of many interesting fields of research.