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dc.contributor.authorMizrachi, Sarah
dc.date.accessioned2018-11-12T20:25:51Z
dc.date.available2018-11-12T20:25:51Z
dc.date.issued2014-04
dc.identifier.urihttps://hdl.handle.net/20.500.12202/4209
dc.identifier.urihttps://ezproxy.yu.edu/login?url=https://repository.yu.edu/handle/20.500.12202/4209
dc.descriptionThe file is restricted for YU community access only.
dc.description.abstractThe directed movement of particles down a temperature gradient, a phenomenon known as thermophoresis, has long been observed and recorded. However, only recently has thermophoresis been put to use in the laboratory for a variety of biophysical applications. The most prominent and successful of these applications include detection of biochemical interactions, analysis of molecular thermal denaturation, and the study of the origin of biomolecules within hydrothermal pores. To detect biochemical interactions, microscale thermophoresis (MST) is performed by setting up a temperature gradient in solution and detecting changes in thermal diffusion induced by binding events. To analyze thermal denaturation of molecules, the molecular movement along a temperature gradient is compared at a range of temperatures and a change in the molecular movement is detected at the melting temperature. Finally, thermophoresis provides insight into the origin of life as it offers a putative explanation for the accumulation and replication of pre-biotic molecules. These novel applications of thermophoresis, at the intersection of the physical and biological sciences, have unique advantages compared to other techniques and hold great potential in the study of many interesting fields of research.en_US
dc.description.sponsorshipS. Daniel Abraham Honors Programen_US
dc.language.isoen_USen_US
dc.publisherStern College for Womenen_US
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
dc.subjectProteins --Denaturation.en_US
dc.subjectThermal diffusivity.en_US
dc.subjectHeat --Transmission.en_US
dc.subjectMolecular dynamics.en_US
dc.titleBiochemical Applications of Thermophoresis: Investigation of Biomolecular Interactions, Thermal Denaturation, and the Origin of Lifeen_US
dc.typeThesisen_US


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Attribution-NonCommercial-NoDerivs 3.0 United States
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 United States