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dc.contributor.authorWang, Emilie-Jeanne Muriel
dc.date.accessioned2018-07-12T17:32:30Z
dc.date.available2018-07-12T17:32:30Z
dc.date.issued2002
dc.identifier.citationSource: Dissertation Abstracts International, Volume: 63-08, Section: B, page: 3580.;Advisors: Harris Goldstein.
dc.identifier.urihttps://yulib002.mc.yu.edu/login?url=http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqm&rft_dat=xri:pqdiss:3063255
dc.identifier.urihttps://hdl.handle.net/20.500.12202/596
dc.description.abstractThe pathogenesis of HIV-1 infection and the evaluation of therapeutics and vaccines can best be studied in vivo. Previously constructed mice were transgenic for expression of the cellular receptors for HIV-1, human CD4 and CCR5, under the control of the T cell-specific Lck promoter. Although splenocytes from these mice were susceptible to in vitro HIV-1 infection, the limitation of HIV-1-susceptible cells to T cells precluded the development of sustained in vivo infection. Therefore we expanded the range of susceptible cells to include monocytes/macrophages and dendritic cells by making transgenic mice with cell-specific promoters. The resulting mice were transgenic for human CD4 and CCR5 expression under the control of either the dendritic cell-specific CD11c promoter or the monocyte/macrophage-specific CD11b promoter and expressed the receptors in a cell-specific manner. In addition, the CD11c/Lck-hCD4/CCR5 mice were capable of sustaining HIV-1 infection after intrasplenic injection of virus. We bypassed the restricted entry of HIV-1 into mouse cells to focus on the post-integration phase of HIV-1 replication by constructing mice that were transgenic for a full-length infectious proviral clone of an M-tropic HIV-1 isolate (HIV-1JR-CSF) and established that the mice displayed plasma viremia. Since JR-CSF transgenic mouse cells were capable of infecting human peripheral blood mononuclear cells, we used them to develop an in vivo model to study HIV-1-infected cell reservoirs. After transfer of JR-CSF leukocytes into SCID mice that had been implanted with human thymic tissue (hu-thy/liv-SCID), we showed that treatment with highly active anti-retroviral therapy (HAART) could protect the implant from infection. After the cessation of HAART, the recipient mouse implants became HIV-1-infected by the persistent transferred JR-CSF cells. This model permits the use of defined cellular populations from the JR-CSF mice to evaluate the capacity of therapeutic interventions aimed at depleting persistent HIV-1 reservoirs. Brain microglia are the major cellular location of HIV-1 replication in the CNS and a potential reservoir of persistently infected cells. To ascertain whether JR-CSF transgenic microglia could be useful in evaluating the contribution of microglia to HIV-1-mediated disease in the CNS, we demonstrated that JR-CSF microglia produced intact virions that were infectious in vitro and in vivo.
dc.publisherProQuest Dissertations & Theses
dc.subjectMicrobiology.
dc.subjectImmunology.
dc.subjectGenetics.
dc.titleOf virus in mice: Defining transgenic mouse models for HIV-1 research
dc.typeDissertation


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