Influence of metals on light emission from chimeric calcium-regulated photoproteins
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Abstract
Our findings revealed that the novel chimeric CaPh, when linked to streptavidin, is capable of emitting light. Our investigation into metal ions demonstrated that different metals affect light emission in varied ways. Specifically, Mg2+ was found to inhibit light emission, indicating the necessity to consider its effects when developing molecular probes for cellular use. Conversely, Sr2+ could initiate light emission even in the absence of Ca2+ and produced light emission similar to Ca2+ when present. Meanwhile, Zn2+ and Cd2+ were observed to have no significant impact on the CaPh’s light emission. Regarding the attachment of streptavidin to either the N or C termini of the CaPh, our results showed that attaching streptavidin to the N-terminus resulted in significantly higher light emission upon Ca2+ addition than attachment at the C-terminus. Therefore, the Nterminus version of the chimeric protein was more extensively studied and analyzed. This suggests that chimeric CaPh with streptavidin attached to the N-terminus serves as an effective component in developing molecular probes with high signal-to-noise ratios. (from Conclusion)