The enzymatic nature of fungal bioluminescence

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dc.contributor.authorOliveira, Anderson G.
dc.contributor.authorStevani, Cassius V.
dc.contributor.orcid0000-0003-1752-0726en_US
dc.date.accessioned2023-12-07T21:54:48Z
dc.date.available2023-12-07T21:54:48Z
dc.date.issued2009-10
dc.descriptionScholarly article / Open accessen_US
dc.description.abstractThe uncertainty about the possible involvement of a luciferase in fungal bioluminescence has not only hindered the understanding of its biochemistry but also delayed the characterization of its constituents. The present report describes how in vitro light emission can be obtained enzymatically from the cold and hot extracts assay using different species of fungi, which also indicates a common mechanism for all these organisms. Kinetic data suggest a consecutive two-step enzymatic mechanism and corroborate the enzymatic proposal of Airth and Foerster. Finally, overlapping of light emission spectra from the fungal bioluminescence and the in vitro assay confirm that this reaction is the same one that occurs in live fungi.en_US
dc.description.sponsorshipAcknowledgements We are indebted to the Instituto Florestal for allowing us to collect fruiting bodies in the Parque Estadual Tur´ıstico do Alto Ribeira (PETAR) and to Prof. Dr J. W. Hastings and Dr Th´er`ese Wilson (Harvard University) and Prof. Dr Etelvino J. H. Bechara (IQ-USP) for their critical reading of this manuscript, their experimental advice and helpful discussions. Special thanks are due to Prof. Dr Roberto M. Torresi (IQ-USP) for the use of his glove box. Financial support of this work was provided by the Fundac¸ ˜ao de Amparo `a Pesquisa do Estado de S˜ao Paulo (FAPESP) under grant numbers 01/04753-6, 06/53628-3 and 04/13856-1.en_US
dc.identifier.citationOliveira, A. G., & Stavani, C. V. (2009). The enzymatic nature of fungal bioluminescence. Photochem. Photobiol. Sci. 8 (10), 1416-1421en_US
dc.identifier.doihttps://doi.org/10.1039/b908982aen_US
dc.identifier.issn1474-9092 (Electronic) Linking ISSN: 1474905X
dc.identifier.urihttps://hdl.handle.net/20.500.12202/9610
dc.language.isoen_USen_US
dc.publisherThe Royal Society of Chemistryen_US
dc.relation.ispartofseriesPhotochem. Photobiol. Sci.;8(10)
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
dc.subjectFungi/enzymologyen_US
dc.subjectCell-Free System/metabolismen_US
dc.subjectCold Temperatureen_US
dc.subjectFungi/metabolismen_US
dc.subjectHot Temperatureen_US
dc.subjectKineticsen_US
dc.subjectLuminescenceen_US
dc.subjectLuminescent Measurementsen_US
dc.titleThe enzymatic nature of fungal bioluminescenceen_US
dc.typeArticleen_US
local.yu.facultypagehttps://www.yu.edu/faculty/pages/oliveira-andersonen_US

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