Please use this identifier to cite or link to this item:
https://hdl.handle.net/20.500.12202/10104
Title: | Combover interacts with the axonemal component Rsp3 and is required for Drosophila sperm individualization |
Authors: | Steinhauer, Josefa Statman, Benjamin Fagan, Jeremy K. Borck, Jacob Surabhi, Satya Yarikipati, Prathibha Edelman, Daniel Jenny, Andreas 0000-0002-0960-5271 |
Keywords: | Phenotype* Axoneme/*metabolism Drosophila Proteins/*metabolism Drosophila melanogaster/*metabolism Intracellular Signaling Peptides and Proteins/*metabolism Nerve Tissue Proteins/*metabolism Spermatogenesis/*physiology Actins/metabolism Genetically Modified Cell Polarity/genetics Drosophila Proteins/genetics Gene Knockdown Techniques Flagella/metabolism Intracellular Signaling Peptides and Proteins/genetics Nerve Tissue Proteins/genetics Sperm Tail/metabolism Spermatids/metabolism Testis/metabolism rho-Associated Kinases/genetics rho-Associated Kinases/metabolism Spermatogenesis Individualization Planar cell polarity |
Issue Date: | 2019 |
Publisher: | Cambridge Eng : Company Of Biologists Limited |
Citation: | Stein, G. L., Berkmann, C. S., & Pollak, B. (2019). What are social work students being taught about palliative care? Palliative & Supportive Care, 17(5),536-541. doi:https://doi.org/10.1017/S1478951518001049x |
Series/Report no.: | Palliative & Supportive Care;17(5) |
Abstract: | Gamete formation is key to survival of higher organisms. In male animals, spermatogenesis gives rise to interconnected spermatids that differentiate and individualize into mature sperm, each tightly enclosed by a plasma membrane. In Drosophila melanogaster , individualization of sister spermatids requires the formation of specialized actin cones that synchronously move along the sperm tails, removing inter-spermatid bridges and most of the cytoplasm. Here, we show that Combover (Cmb), originally identified as an effector of planar cell polarity (PCP) under control of Rho kinase, is essential for sperm individualization. cmb mutants are male sterile, with actin cones that fail to move in a synchronized manner along the flagella, despite being correctly formed and polarized initially. These defects are germline autonomous, independent of PCP genes, and can be rescued by wild-type Cmb, but not by a version of Cmb in which known Rho kinase phosphorylation sites are mutated. Furthermore, Cmb binds to the axonemal component Radial spoke protein 3, knockdown of which causes similar individualization defects, suggesting that Cmb coordinates the individualization machinery with the microtubular axonemes. |
Description: | Scholarly article / Open access |
URI: | https://ezproxy.yu.edu/login?url=https://search.ebscohost.com/login.aspx?direct=true&AuthType=ip,sso&db=cmedm&AN=31391193&site=eds-live&scope=site https://hdl.handle.net/20.500.12202/10104 |
ISSN: | : 09501991 |
Appears in Collections: | Yeshiva College: Faculty Publications |
Files in This Item:
File | Description | Size | Format | |
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Steinhauer 2019 OA Combover interacts.pdf | 928.99 kB | Adobe PDF | View/Open |
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