Please use this identifier to cite or link to this item:
https://hdl.handle.net/20.500.12202/9605
Title: | Genetically encodable bioluminescent system from fungi |
Authors: | Oliveira, Anderson G. Kotlobay, Alexey A. Sarkisyan, Karen S. Mokrushina, Yuliana A. Marcet-Houben, Marina Serebrovskaya, Ekaterina O. Markina, Nadezhda M. Somermeyer, Louisa Gonzalez Gorokhovatsky, Andrey Y. Vvedensky, Andrey Purtov, Konstantin V. Petushkov, Valentin N. Rodionova, Natalja S. 0000-0003-1752-0726 |
Keywords: | bioluminescence fungal luciferinbiosynthesis fungal luciferase |
Issue Date: | 2018 |
Citation: | Kotlobay, A. A., Sarkisyan, K. S., Mokrushina, Y. A., Marcet-Houben, M., Serebrovskaya, E. O., Markina, N. M., Somermeyer, L. G., Gorokhovatsky, A. Y., Vvedensky, A., Purtov, K. V., Petushkov, V. N., Rodionova, N. S., Chepurnyh, T. V., Fakhranurova, L. I., Guglya, E. B., Ziganshin, R., Tsarkova, A. S., Kaskova, Z. M., Shender, V., … Yampolsky, I. V. (2018). Genetically encodable bioluminescent system from fungi. Proceedings of the National Academy of Sciences of the United States of America, 115(50), 12728–12732. |
Series/Report no.: | Proceedings of the National Academy of Sciences of the United States of America;115(50) |
Abstract: | Bioluminescence is found across the entire tree of life, conferring a spectacular set of visually oriented functions from attracting mates to scaring off predators. Half a dozen different luciferins, molecules that emit light when enzymatically oxidized, are known. However, just one biochemical pathway for luciferin biosynthesis has been described in full, which is found only in bacteria. Here, we report identification of the fungal luciferase and three other key enzymes that together form the biosynthetic cycle of the fungal luciferin from caffeic acid, a simple and widespread metabolite. Introduction of the identified genes into the genome of the yeast Pichia pastoris along with caffeic acid biosynthesis genes resulted in a strain that is autoluminescent in standard media. We analyzed evolution of the enzymes of the luciferin biosynthesis cycle and found that fungal bioluminescence emerged through a series of events that included two indepen- dent gene duplications. The retention of the duplicated enzymes of the luciferin pathway in nonluminescent fungi shows that the gene duplication was followed by functional sequence divergence of enzymes of at least one gene in the biosynthetic pathway and suggests that the evolution of fungal bioluminescence proceeded through several closely related stepping stone nonluminescent biochemical reactions with adaptive roles. The availability of a complete eukaryotic luciferin biosynthesis pathway provides sev- eral applications in biomedicine and bioengineering. |
Description: | Scholarly article / OA |
URI: | https://ezproxy.yu.edu/login?url=https://search.ebscohost.com/login.aspx?direct=true&AuthType=ip,sso&db=edsjsr&AN=edsjsr.26580150&site=eds-live&scope=site https://hdl.handle.net/20.500.12202/9605 |
Appears in Collections: | Stern College for Women -- Faculty Publications |
Files in This Item:
File | Description | Size | Format | |
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Oliveira OA 2018 Genetically encodable 1803615115.full.pdf | 1.71 MB | Adobe PDF | View/Open |
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